Endocrine Abstracts

Trans-differentiation of osteoblasts to adipocytes may in part be responsible for diseases such as osteoporosis and arthritis. The processes involved are however complex and largely unknown. Our previous data showed that the adenosine A2b receptor (A2bR) is important for human osteoprogenitor cell function. In this study, we investigated the expression of adenosine receptors in an osteoblast cell line (7F2) and during its differentiation into adipocytes (7F2A).<p class="ab...

Osteoblasts and adipocytes are derived from mesenchymal stem cells (MSC), although the mechanisms involved are not fully understood. Our previous findings showed that MSCs express functional adenosine receptors (ARs) and that adenosine stimulates osteoblastogenesis. Here we describe changes in AR expression as MSCs differentiate into osteoblasts and adipocytes, and provide evidence that ARs are involved in these differentiation pathways.Rat MSCs were dif...

Pluripotent mesenchymal stem cells (MSCs) have the capacity to differentiate into osteoblasts, adipocytes and chondrocytes and are characterised by expression of CD105, CD90 and CD73. CD73 is the enzyme that dephosphorylates AMP to adenosine. The role of adenosine in MSCs is unknown and we have thus investigated whether it plays a role in the differentiation process to osteoblasts.MSCs, obtained from the bone marrow of 10 week old male rats, were expande...